logo
AAT Bioquest

FAQ

Posted on March 18, 2024
What is fluorescence resonance energy transfer used for?
Antibodies and Proteomics Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological Probes


Posted on March 18, 2024
What are the advantages of viable cell count?
Chemical ReagentsCell Viability AssaysCell countingClassic DyesLive or DeadPhysiological Probes


Posted on March 18, 2024
What are the disadvantages of viable cell count?
Chemical ReagentsCell Viability AssaysCell countingClassic DyesPhysiological Probes


Posted on March 12, 2024
What are the similarities between regressive and progressive staining?
Chemical ReagentsCellular Structures and OrganellesClassic DyesNucleic AcidsNucleusPhysiological Probes


Posted on March 12, 2024
What are the differences between regressive and progressive staining?
Chemical ReagentsCellular Structures and OrganellesClassic DyesNucleic AcidsNucleusPhysiological Probes


Posted on March 12, 2024
What does cell viability mean?
Chemical ReagentsCell Viability AssaysCellular ProcessesCellular Structures and OrganellesClassic DyesPhysiological Probes


Posted on January 16, 2024
What are the advantages of using fluorescent dyes to label cells?
BioconjugationCellular Structures and OrganellesClassic DyesFluoresceinsFluorescence


Posted on September 15, 2023
What are the characteristics of ionic bonds?
Bioconjugation Chemical ReagentsClassic DyesCrosslinkers


Posted on July 6, 2023
Which enzyme is commonly called a molecular scissor?
Bioconjugation Chemical ReagentsClassic DyesDNAEnzymesPhysiological Probes


Posted on July 6, 2023
What should I do to prevent star activity?
Bioconjugation Chemical ReagentsClassic DyesDNAEnzymesPhysiological Probes


Posted on July 6, 2023
What causes star activity in restriction enzyme digestions?
Bioconjugation Chemical ReagentsClassic DyesDNAEnzymesPhysiological Probes


Posted on July 6, 2023
What are the applications of restriction enzymes?
Bioconjugation Chemical ReagentsClassic DyesDNAEnzymesPhysiological Probes


Posted on July 6, 2023
What are the factors that affect the activity of restriction enzymes?
Bioconjugation Chemical ReagentsClassic DyesEnzymesPhysiological Probes


Posted on June 21, 2023
What are the pros of membrane asymmetry cell viability assays?
Annexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesChemical ReagentsClassic Dyes


Posted on June 21, 2023
What are the cons of membrane asymmetry cell viability assays?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell Viability AssaysCellular ProcessesClassic Dyes


Posted on May 9, 2023
What advantages does FACS have over MACS?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on May 9, 2023
How do I choose the best cell separation method for my research?
BioconjugationCellular ProcessesClassic DyesFlow Cytometry ReagentsSpectral Flow Cytometry


Posted on April 26, 2023
What's the importance of pH in biological systems?
Chemical ReagentsClassic DyesFluoresceinsIntracellular pHPhysiological Probes


Posted on April 26, 2023
What is the pH for an acidic substance?
Chemical ReagentsClassic DyesFluoresceinsIntracellular pHPhysiological Probes


Posted on March 16, 2023
What is the principle of indirect staining?
Classic Dyesbacteria


Posted on March 9, 2023
What are the most commonly used biological stains?
Classic DyesDAPIPhysiological Probesethidium bromide


Posted on January 30, 2023
What are the properties of glycine?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 27, 2023
Is glycine a weak acid?
Bioconjugation Chemical ReagentsClassic DyesPolymerase Chain Reaction (PCR)


Posted on January 25, 2023
What are the factors affecting fluorescence?
Chemical ReagentsClassic DyesPhysiological Probes


Posted on January 13, 2023
What are the differences between static quenching and dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the applications of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesDNADNA and RNA QuantitationFluorescencePhysiological Probes


Posted on January 13, 2023
What is the mechanism of dynamic quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on January 13, 2023
What are the types of fluorescence quenching?
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesFluorescencePhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes


Posted on November 16, 2022
What are the single membrane organelles?
Chemical ReagentsCellular Structures and OrganellesClassic DyesEndoplasmic Reticulum (ER)Golgi ApparatusLysosomesPhysiological Probes


Posted on November 16, 2022
What are cellular membranes made of?
Chemical ReagentsCell Plasma Membrane probesCellular Structures and OrganellesClassic DyesPhysiological ProbesPlasma Membrane


Posted on November 16, 2022
What is the largest membrane-bound organelle in eukaryotic cells?
Chemical ReagentsCellular Structures and OrganellesClassic DyesNucleic AcidsNucleusPhysiological Probes


Posted on November 16, 2022
What are the similarities between cell organelles and cell inclusions?
Chemical ReagentsCellular Structures and OrganellesClassic DyesPhysiological ProbesPlasma Membrane


Posted on November 16, 2022
What are the differences between cell organelles and cell inclusions?
Chemical ReagentsCellular Structures and OrganellesClassic DyesEndoplasmic Reticulum (ER)Golgi ApparatusLysosomesMitochondriaNucleusPhysiological Probes


Posted on November 16, 2022
What are the general features of inclusion bodies?
Chemical ReagentsCellular Structures and OrganellesClassic DyesOrganellesPhysiological Probes


Posted on November 16, 2022
Can ribosomes be considered inclusion bodies?
Chemical ReagentsCellular Structures and OrganellesClassic DyesOrganellesPhysiological Probes


Posted on November 14, 2022
What is the difference between Black Hole Quencher (BHQ) dye and TAMRA?
Bioconjugation Chemical ReagentsClassic DyesDNA and RNA QuantitationFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)


Posted on November 14, 2022
What are the differences between GFP and YFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What activates GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the applications of GFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingChemical ReagentsClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What's the relationship between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the differences between GFP and EGFP?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesGreen FluorescenceProtein


Posted on November 14, 2022
What are the types of fluorophores?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic DyesFluoresceinsProteinRhodamines and Rhodamine Derivatives


Posted on November 10, 2022
What are the applications of TRITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
How are FITC signals detected?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFluoresceinsImmunohistochemistry (IHC)amine-reactiveimmunocytochemistry


Posted on November 10, 2022
How do I store FITC-dextran?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the different isomers of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What are the physical properties of FITC?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
How do I dissolve a FITC-dextran?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC hydrophobic?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryimmunocytochemistry


Posted on November 10, 2022
Is FITC soluble in water?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
Is FITC a fluorophore?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsImmunohistochemistry (IHC)Spectral Flow Cytometryamine-reactiveimmunocytochemistry


Posted on November 10, 2022
What does FITC bind to?
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization


Posted on November 10, 2022
What are the common fluorescein applications?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsFluoresceinsPhysiological ProbesSpectral Flow Cytometryimmunofluorescence


Posted on November 8, 2022
Are all zwitterions pH neutral?
Chemical ReagentsBioconjugationClassic DyesDye to Peptide Conjugation


Posted on November 7, 2022
Why are Annexin V conjugates and 7-AAD used together?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell Cycle AssaysCell Viability AssaysCellular ProcessesCellular Structures and OrganellesClassic DyesFluoresceinsNucleusannexin V


Posted on October 24, 2022
What are the different types of ligands?
Chemical ReagentsCell Structures and OrganellesClassic DyesNucleusPhysiological Probes


Posted on October 14, 2022
Is coumarin legal?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Where does coumarin come from?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Are coumarins water-soluble?
Chemical ReagentsBioconjugationClassic DyesCoumarins


Posted on October 14, 2022
Is coumarin a natural chemical?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Is there a better dye than AMCA?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What are coumarin derivatives used for?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Are coumarins useful for immunofluorescence?
Chemical Reagents Enzyme-Linked Immunosorbent Assay (ELISA)BioconjugationClassic DyesCoumarins


Posted on October 14, 2022
Are coumarin dyes pH sensitive?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What color do coumarins fluoresce?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What are the applications of coumarin dyes?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What does 'coumarin nucleus' mean?
Bioconjugation Chemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
What is coumarin used for?
BioconjugationChemical ReagentsClassic DyesCoumarins


Posted on October 14, 2022
Is coumarin a carcinogen?
Chemical ReagentsBioconjugationClassic DyesCoumarins


Posted on October 14, 2022
What are the steps of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingAntibody and ProteomicsClassic Dyes


Posted on October 14, 2022
What are the principles of IR spectroscopy/vibrational spectroscopy?
Antibody and Protein LabelingAntibody and ProteomicsBioconjugationClassic Dyes


Posted on September 1, 2022
What are the purposes of a fluorescent marker?
BioconjugationCell Structures and OrganellesCell Viability AssaysCellular ProcessesClassic DyesCluster of Differentiation (CD Antibodies)Cyanines


Posted on September 1, 2022
What are the differences between flow cytometry and FACS?
BioconjugationCell Structures and OrganellesCellular ProcessesClassic DyesFlow Cytometry Reagents


Posted on August 30, 2022
What are the principles of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 30, 2022
What are the steps of magnetic resonance spectroscopy?
Antibodies and Proteomics Bioconjugation Chemical ReagentsClassic Dyesspectrophotometer


Posted on August 19, 2022
What is the principle of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the uses of fluorescence spectroscopy?
BioconjugationAntibody and Protein LabelingClassic DyesFluorescence


Posted on August 19, 2022
What are the uses of IR spectroscopy/vibrational spectroscopy?
BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the principles of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 19, 2022
What are the steps of mass spectroscopy?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingClassic Dyes


Posted on August 11, 2022
What are the B cell surface markers?
Cluster of Differentiation (CD Antibodies)Flow Cytometry ReagentsPhysiological ProbesClassic Dyes


Posted on May 24, 2022
What is the difference between mass spectroscopy and mass spectrometry?
Classic DyesBioconjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on May 13, 2022
How do I tell if it's a physical or chemical change?
Classic DyesBioconjugationPhysiological Probes


Posted on May 12, 2022
Why do some chemical bonds need more energy to break?
Chemical ReagentsClassic DyesCrosslinkersBioconjugation


Posted on April 25, 2022
What are the branches of chemistry and their definition?
Chemical ReagentsClassic Dyes


Posted on April 25, 2022
What are the differences between chemical change and physical change?
Chemical ReagentsClassic Dyes


Posted on April 25, 2022
What are the common grades for chemicals and reagents?
Chemical ReagentsClassic Dyes


Posted on April 22, 2022
What are the primary types of chemical bonds?
Chemical ReagentsClassic Dyes


Posted on April 19, 2022
What are examples of the common physical changes?
Classic DyesPhysiological Probes


Posted on April 19, 2022
What are the basic types of chemical reactions?
Chemical ReagentsPhysiological ProbesClassic Dyes


Posted on March 16, 2022
What are the two common approaches used in column chromatography?
Chemical ReagentsClassic DyesDyes by Functional Groups


Posted on March 16, 2022
What are the common methods for protein purification?
Chemical ReagentsClassic DyesAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 3, 2022
What are the differences between a standard PCR and RT PCR?
Reverse Transcription PCR (RT-PCR)Polymerase Chain Reaction (PCR)Chemical ReagentsClassic DyesDNA and RNA Quantitation


Posted on December 13, 2021
What are coumarin dyes used for?
CoumarinsClassic DyesChemical Reagents


Posted on October 29, 2021
What’s the difference between FITC and FAM?
FluoresceinsPeptide and Oligonucleotide LabelingClassic DyesBioconjugation


Posted on October 27, 2021
How do I choose the right fluorophore?
Antibodies and ProteomicsiFluor Dyes and KitsClassic Dyes


Posted on October 1, 2021
Which rhodamines are used in biological research laboratories?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups


Posted on October 1, 2021
Is Rhodamine 6G soluble in water?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups


Posted on October 1, 2021
What is Rhodamine B used for?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups


Posted on October 1, 2021
What is the difference between Rhodamine 123 and Rhodamine B?
Chemical ReagentsClassic DyesDyes by Functional GroupsRhodamines and Rhodamine Derivatives


Posted on October 1, 2021
Is Rhodamine B an amphoteric dye?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Groups


Posted on September 30, 2021
Is rhodamine hydrophilic or hydrophobic?
Rhodamines and Rhodamine DerivativesChemical ReagentsDyes by Functional GroupsClassic Dyes


Posted on September 29, 2021
What is the difference between Rhodamine B and Rhodamine 6G?
Rhodamines and Rhodamine DerivativesClassic DyesChemical ReagentsDyes by Functional Group


Posted on September 29, 2021
What wavelength does rhodamine B absorb?
Classic DyesChemical ReagentsDyes by Functional GroupRhodamines and Rhodamine Derivatives


Posted on September 29, 2021
What is the quantum yield of rhodamine B?
Classic DyesChemical ReagentsDyes by Functional Group


Posted on September 29, 2021
What other names is rhodamine B called?
Chemical ReagentsClassic DyesDye by Functional Group


Posted on September 29, 2021
Are rhodamines toxic?
Classic DyesChemical ReagentsDyes by Functional GroupRhodamines and Rhodamine Derivatives


Posted on September 29, 2021
Are rhodamines pH-dependent?
Classic DyesChemical ReagentsDyes by Functional Group


Posted on August 16, 2021
What is SYPRO Ruby stain?
CyaninesClassic DyesPeptide and Oligonucleotide LabelingDNA and RNA Quantitation


Posted on August 12, 2021
Is indocyanine green (ICG) used for small animal in vivo imaging?
BioconjugationChemical ReagentsClassic Dyes


Posted on August 12, 2021
Does indocyanine green (ICG) have a charge?
Chemical ReagentsClassic Dyes


Posted on August 11, 2021
Is indocyanine green (ICG) FDA approved?
Chemical ReagentsClassic DyesDyes by Functional Group


Posted on August 11, 2021
What is indocyanine green (ICG)?
Classic DyesChemical Reagents


Posted on August 10, 2021
How long does indocyanine green (ICG) stay in tissues after being injected?
Chemical ReagentsDyes by Functional GroupClassic Dyes


Posted on July 28, 2021
What color is Cy7?
CyaninesClassic DyesAntibody and Protein Labeling


Posted on July 27, 2021
Can Cy3 be used in flow cytometry?
Chemical ReagentsCycanineClassic Dyes


Posted on July 27, 2021
Are any of the cyanine dyes infrared?
CyaninesClassic DyesChemical Reagents


Posted on July 27, 2021
Which cyanine dyes can be used for in vivo imaging applications?
CyaninesClassic DyesChemical ReagentsPeptide and Oligonucleotide Labeling


Posted on July 20, 2021
What is the difference between Cy5 and Cy5.5?
Classic DyesCyaninesChemical Reagents


Posted on July 1, 2021
What color is Cy3?
BioconjugationClassic DyesCynines


Posted on July 1, 2021
Is PE Cy7 photosensitive?
PE and APCClassic DyesBioconjugation


Posted on July 1, 2021
Is Cy3 a fluorophore?
CyaninesClassic DyesBioconjugation


Posted on July 1, 2021
Can cyanine dyes be used in tandem with other dyes?
PE and APCCyaninesClassic Dyes


Posted on July 1, 2021
Is Cy5 hydrophobic?
CyaninesClassic DyesBioconjugation


Posted on July 1, 2021
What are cyanine dyes used for?
CyaninesClassic DyesDye to Peptide Conjugation


Posted on March 17, 2021
What does FRET mean?
Förster Resonance Energy Transfer (FRET)Peptide and Oligonucleotide LabelingChemical ReagentsClassic Dyes


Posted on March 16, 2021
What is FRET imaging?
Classic DyesChemical ReagentsFörster Resonance Energy Transfer (FRET)


Posted on March 16, 2021
What is FRET technique?
Classic DyesChemical Reagents


Posted on March 16, 2021
What makes a good fluorophore?
Classic DyesChemical Reagents


Posted on March 16, 2021
Is a fluorophore and a fluorochrome the same thing?
Classic DyesChemical Reagents


Posted on January 22, 2021
Is DNA methylation reversible?
Classic DyesNucleic Acids


Posted on November 30, 2020
How do I calculate PCR efficiency?
DNA and RNAChemical ReagentsClassic Dyes


Posted on November 25, 2020
How do I evaluate the performance of a real-time PCR reaction?
Classic DyesDNA and RNAChemical Reagents


Posted on October 7, 2020
What is the molecular weight of 3-cyano-7-hydroxycoumarin?
CoumarinsClassic Dyes


Posted on June 1, 2020
Why do some absorbing compounds fluoresce but others do not?
Chemical reagentsClassic DyesFluorescencePhysiological Probes


Posted on June 1, 2020
What is the mitosis phase in a cell cycle and what are the phases of mitosis phase?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationCellular Structures and OrganellesClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
How long is the duration of each phase in a cell cycle?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
How does BrdU labeling work?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on June 1, 2020
What is the advantage of tandem dyes in flow cytometry?
Bioconjugation Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCSpectral Flow Cytometryflow cytometry


Posted on June 1, 2020
What is the difference between cell proliferation and cell growth?
Chemical ReagentsCell Cycle and ProliferationCell Proliferation AssaysCellular ProcessesClassic Dyescell proliferation


Posted on June 1, 2020
What is cell division and types?
Chemical ReagentsCell Cycle AnalysisCell Cycle AssaysCell Cycle and ProliferationClassic DyesFlow Cytometry ReagentsFluoresceinsflow cytometry


Posted on May 18, 2020
What is FSC and SSC in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometry


Posted on May 18, 2020
Are there upgraded trypan blue derivatives for cell viability testing?
Chemical ReagentsCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesFluoresceinsFluorescencePhysiological Probes


Posted on May 18, 2020
How do you detect apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 18, 2020
What is the essential difference between apoptosis and necrosis?
Apoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessClassic DyesPhysiological Probesannexin Vcell death


Posted on May 18, 2020
How do you identify stages of apoptosis?
Chemical ReagentsAnnexin-V StainingApoptosis and NecrosisCellular ProcessesCellular Structures and OrganellesClassic DyesPhysiological ProbesTUNELannexin V


Posted on May 18, 2020
What is the difference between Side Scatter Pulse Width (SSC-w) and Side Scatter Pulse Height (SSC-h)?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsImmunohistochemistry (IHC)Physiological ProbesSpectral Flow Cytometryflow cytometrymultiplex


Posted on May 9, 2020
Are Annexin V-FITC+/PI+ cells classified as late-apoptotic cells or necrotic cells?
Chemical ReagentsAV-FITCAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesPropidium iodide (PI)annexin V


Posted on May 9, 2020
Can apoptosis be detected by cell cycle studies?
Chemical ReagentsAnnexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysCellular ProcessesClassic DyesTUNELannexin V


Posted on May 9, 2020
Which probe should I use to evaluate change in membrane potential, JC-1 or TMRE?
Chemical ReagentsCellular ProcessesClassic DyesJC-1JC-10Membrane Potential and ChannelsMitochondriamitochondria membrane potential


Posted on May 9, 2020
What stain should I use to analyze apoptosis in flow cytometry?
Annexin-V StainingApoptosisApoptosis and NecrosisCell ViabilityCell Viability AssaysClassic DyesFlow Cytometry ReagentsFluoresceinsannexin Vflow cytometry


Posted on May 9, 2020
What is the number of events in flow cytometry?
Chemical ReagentsClassic DyesFlow Cytometry ReagentsPE and APCPhysiological ProbesSpectral Flow Cytometryflow cytometryiFluor Dyes and Kitsmultiplex


Posted on April 24, 2020
What is end-point RT-PCR?
Chemical ReagentsClassic DyesDNA and RNA QuantitationDNA and RNAFluorescencePCRPhysiological ProbesPolymerase Chain Reaction (PCR)RNAReverse Transcription PCR (RT-PCR)ethidium bromide


Posted on April 17, 2020
What is IHC double staining?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Chemical ReagentsClassic DyesIHCPhysiological Probes


Posted on April 17, 2020
Which is the best conditions for methanol fixation of microtubules?
Bioconjugation Chemical ReagentsClassic DyesFixationPhysiological Probesfixed cells


Posted on April 7, 2020
What rhodamine dye do you have for automated DNA sequencing and nucleotide labeling?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesDNA sequencingFluoresceinsRhodamines and Rhodamine DerivativesPhysiological Probes


Posted on April 7, 2020
Share several ways of labeling an oligonucleotide with fluorescein like 6-FAM/6-HEX/6-TET phosphoramidite?
Chemical Reagents Peptide and Oligonucleotide LabelingClassic DyesFluoresceinsPhysiological Probes


Posted on April 2, 2020
What is the role of magnesium in cell metabolism?
Chemical ReagentsCellular MetabolismCellular ProcessesClassic DyesFluoresceinsmagnesium